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Reaction mixtures (100 μl) containing 11 μg enzymes, 1 μM iAs3+, 1 mM SAM and 7 mM GSH in PBS (25 mM, pH 7.0) were incubated at 37°C for 2 h and analyzed using HPLC-ICP-MS. The percents of arsenic species (iAs/TAs, MMA/TAs and DMA/TAs) and the two indices (FMR and SMR) of mutant C85S are shown in Figures 3a and 3b. Values are the averages ± S.D. of three independent experiments performed using three independently purified proteins.
