Figure 6. Catalytic capacity of MMA³⁺ for WT-hAS3MT and hAS3MT cysteine mutants.

Reaction mixtures (100 μl) containing 11 μg enzymes, 1 μM MMA³⁺, 1 mM SAM, and 7 mM GSH/1 mM TCEP/100 μM DHLA in PBS (25 mM, pH 7.0) were incubated at 37°C for 2 h and analyzed by HPLC-ICP-MS. a, b, and c represent three reductants: 7 mM GSH, 1 mM TCEP, and 100 μM DHLA, respectively. Values are the means ± S.D. of three independent experiments.