Table 1. Primers used for site-directed mutagenesis.

	Primer sequence
C32S	−    5′-GTGGTCACAGAACCGTTGG-3′
C61S	−    5′-CCAGACCGGAGCCATAATATCTTAGGG-3′
C85S	+    5′-GTGGTAGAGATTCCTATGTACTTAGCC-3′−    5′-GGCTAAGTACATAGGAATCTCTACCAC-3′
Whole	+    5′-CGGGATATCATGGCTGCACTTCGTGAC-3′−    5′-CGGGTCGACTTAGTGATGGTGATG-3′

The “Whole” in Table 1 refers upstream and downstream primers for full length gene of the hAS3MT.