Table 3. Secondary structures of WT-hAS3MT and the mutants estimated using CD spectroscopy.

	α-helix (%)	β-pleated (%)	β-turn (%)	Random (%)
C32S	31.7±1.2	30.4±1.1	14.0±0.7	23.6±0.5
C61S	33.5±1.8	36.9±1.6	8.5±0.4	21.1±0.5
C85S	26.5±1.3	31.9±2.2	14.5±0.5	27.1±0.8
WT	29.0±2.2	23.9±1.9	17.9±1.7	29.2±1.4

Values represent the mean ± S.D. of three independent experiments. The parameters were analyzed using the Jasco secondary structure manager with the reference CD data-Yang. jwr in PBS (25 mM, pH 7.0) at room temperature.