Table 4. Secondary structures of WT-hAS3MT and the mutants estimated from ATR-FTIR spectroscopy.
α-helix (%) | β-pleated (%) | β-turn (%) | Random (%) | |
C32S | 32.7±1.5 | 28.2±0.3 | 15.5±0.3 | 23.5±0.8 |
C61S | 31.7±1.6 | 37.7±2.6 | 10.6±0.3 | 20.1±0.6 |
C85S | 27.0±1.2 | 30.1±2.3 | 16.1±0.7 | 26.8±1.1 |
WT | 26.6±3.6 | 20.7±4.6 | 24.2±3.2 | 28.5±4.9 |
Values represent the mean ± S.D. of three independent experiments. The parameters were analyzed using the origin 7.0.