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. 2013 Dec 31;8(12):e84390. doi: 10.1371/journal.pone.0084390

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© 2013 Zhu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cleavage analysis was performed using 5′ RACE. Two bands were amplified and sequenced for Gorai.007G320500 whereas a single band was sequenced for other three genes. Gorai.007G320500 is also a predicted target of ghr-miR482c. The sequences of ghr-miR482d and ghr-miR482d.2 are underlined and showed in italic, respectively. The expected cleavage sites of ghr-miR482 and ghr-miR482.2 were indicated by pink and blue arrows, respectively. The identified cleavage sites are indicated by black arrows with the frequency of cleavage showing on top of the arrows.