Table 1. MS analysis and database searching of the 13 spots with differential phosphorylation level in samples from anti-TG2 treated cells and from control-IgG treated cells.

Spot number	UniProtProtein ID	UniProt ProteinAccession Code	Protein name	MASCOT Score	ProteinMass	Protein pI	UniquepeptideMatches	ProteinCoverage	emPAI
4403	Q53G99	Q53G99_HUMAN	Beta actin variant	635	42080	5.37	11	38.1	1.56
4406	B4DW52	B4DW52_HUMAN	Uncharacterized protein	523	38950	5.19	9	35.2	1.51
4607	P10809	CH60_HUMAN	60 kDa heat shock protein,mitochondrial	453	61187	5.24	9	19.2	0.61
4702	Q53HF2	Q53HF2_HUMAN	Heat shock 70kDa protein 8isoform 2 variant	641	53580	5.62	13	27.4	1.1
4706	E9PNE6	E9PNE6_HUMAN	Uncharacterized protein	185	55170	5.51	4	10	0.3
5407	P06732	KCRM_HUMAN	Creatine kinase M-type	332	43302	6.27	6	22	1.49
5604	Q5SU16	Q5SU16_HUMAN	Beta 5-tubulin	300	50095	4.78	7	16	0.81
5604	Q71U36	TBA1A_HUMAN	Tubulin alpha-1A chain	129	50788	4.94	3	8.2	0.24
6003	P13693	TCTP_HUMAN	Translationally-controlledtumor protein	78	19697	4.84	2	15.7	0.43
6604	Q13885	TBB2A_HUMAN	Tubulin beta-2A chain	667	50274	4.78	12	29.7	1.36
7603	P07237	PDIA1_HUMAN	Protein disulfide-isomerase	362	57480	4.76	7	17.5	0.55
7804	P14625	ENPL_HUMAN	Endoplasmin	1034	92696	4.76	21	27	1.27
8204	P62258	1433E_HUMAN	14-3-3 protein epsilon	492	29326	4.63	9	42.7	1.98
8404	P26641	EF1G_HUMAN	Elongation factor 1-gamma	103	50429	6.25	2	6.2	0.15

Spot number, UniProt protein ID and accession code, protein name, Mascot score, theoretical protein mass and pI values, number of peptide matches, sequence coverage and emPAI values are listed for each protein.