Table 4. Differential expression analysis using DESeq package.
Differentially expressed transcripts with padj<0.1 after dsRNA injection of Pcsut1: | ||||||
Seq_Id | baseMeanA | baseMeanB | foldChange | log2FoldChange | pval | padj |
PcSUT1 | 4804.953616 | 240.8731589 | 0.050130174 | −4.31817696 | 1.26E-16 | 7.31E-15 |
Differentially expressed transcripts with padj<0.1 after dsRNA injection of Pcsut 2: | ||||||
Seq_Id | baseMeanA | baseMeanB | foldChange | log2FoldChange | pval | padj |
PcSUT2 | 8428.420254 | 2031.419654 | 0.241020214 | −2.052773946 | 4.15E-08 | 2.49E-06 |
PcSUT25 | 22.79115587 | 394.3587471 | 17.30314817 | 4.112962644 | 0.003061159 | 0.091834771 |
Differentially expressed transcripts with padj<0.1 after dsRNA injection of Pcsut 6: | ||||||
Seq_Id | baseMeanA | baseMeanB | foldChange | log2FoldChange | pval | Padj |
PcSUT6 | 3380.778995 | 95.65776606 | 0.028294593 | −5.143329799 | 4.20E-14 | 2.56E-12 |
PcSUT25 | 22.79115587 | 995.628527 | 43.68486322 | 5.449061568 | 0.000272434 | 0.008309243 |
baseMeanA: mean of normalized counts value of dsgfp-injected samples. baseMeanB: mean of normalized counts values of dsRNA-gfp-injected, dsRNA-Pcsut1-injected, dsRNA-Pcsut2-injected, dsRNA-Pcsut6-injected samples. Fold-change: baseMeanA compared to baseMeanB. Log2fold-change: logarithm (to base 2) of fold-change values. Pval: p-value for the statistical significance of this change. Padj: p-value adjusted for multiple testing with Benjamini-Hochberg procedure which controls false discovery rate.