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. 2013 Dec 31;8(12):e84340. doi: 10.1371/journal.pone.0084340

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© 2013 Jin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mast cells were derived from bone marrow (BM) and peritoneal cavity (PC) of B6, B6-cma, and DBA/2 mice. Cells were either extracted for assays of total chymase activity (A) or treated with antigen to induce degranulation for determination of secreted chymase activity (B). Specific activity was calculated in reference to total proteins in cell pellets. Error bars denote standard deviation (n≥3). *p<0.0001 in comparison with B6 control mice.