Table 1. Differentially regulated genes from microarray.

Gene symbol	Description	ΔGATA-6	ΔGATA-4	Difference
Timp4	Tissue inhibitor of metalloproteinase 4	−1.18	3.00	3.56
Angptl7	Angiopoietin-like 7	1.05	2.39	2.28
Spink4	serine peptidase inhibitor, Kazal type 4	1.02	2.11	2.07
Gcnt1	Glucosaminyl (N-acetyl) transferase 1	−1.17	2.89	2.03
Ptgfr	Prostaglandin F receptor	−1.00	2.92	1.92
Avpr1a	Arginine vasopressin receptor 1A	1.06	1.58	1.49
Zbtb16	Zinc finger and BTB domaincontaining 16	−1.17	2.10	1.24
Bhlhb9	Basic helix loop helix domain containing class B9	−1.05	2.18	1.23
Scgb1c1	Secretoglobin 1C1	−1.01	2.09	1.10
Gm129	Gene model 129	−1.10	2.00	1.09
Fmo2	Flavin containing monooxygenase 2	−1.10	1.83	0.92
Gsta3	Glutathione S transferase, alpha 3	−1.04	1.81	0.85
Adh1	Alcohol dehydrogenase 1	−1.28	1.61	0.83
Rhobtb1	Rho related BTB domain containing 1	−1.09	1.61	0.69
Tsc22d3	TSC22 domain family 3	−1.05	1.64	0.69
Wee1	Wee 1 homolog	−1.06	1.57	0.63
Pfn2	Profilin 2	−1.02	1.61	0.63
Bc055107	Family with sequence similarity 107, A	−1.13	1.51	0.63
Fstl4	Follistatin like 4	−1.37	1.34	0.61
Fkbp5	FK506 binding protein 5	−1.20	1.40	0.57
Gadd45g	Growth arrest and DNA damageinducible 45γ	−1.06	1.53	0.56
Bcl2l1	BCL2 like 1	−1.08	1.45	0.52
Ifi205	Interferon activated gene 205	−1.15	1.38	0.51
Tchhl1	Trichohyalin like 1	−1.02	1.45	0.47
Thrsp	Thyroid hormone responsiveSPOT14 homolog	−1.46	1.14	0.46
Il33	Interleukin 33	−1.03	1.42	0.45
Trdn	Triadin	−1.26	1.25	0.45
Ankrd10	Ankyrin repeat domain 10	−1.02	1.42	0.44
Ddit4	DNA damage inducible transcript 4	−1.02	1.42	0.44
Tom1l1	Target of myb1 like 1	−1.06	1.38	0.44
Cebpb	CCAAT/enhancer binding protein(C/EBP) beta	−1.08	1.35	0.42
Efna5	Ephrin A5	−1.04	1.38	0.42
Rgs2	Regulator of G-protein signaling 2	−1.01	1.41	0.42
Hey2	Hairy/enhancer of split related withYRPW motif 2	−1.35	1.09	0.35
Drd2	Dopamine receptor 2	1.57	−1.18	0.73
Nppb	Natriuretic peptide precursor type B	1.55	−1.04	0.59
Egr1	Early growth response 1	1.19	−1.62	0.58
Fbn1	Fibrillin 1	1.26	−1.41	0.55
Eg633640	Predicted gene	1.31	−1.30	0.53
Rrad	Ras-related associated with diabetes	1.00	−2.09	0.52
Ptk2b	PTK2 protein tyrosine kinase 2 beta	1.26	−1.35	0.51
Trmt5	TRM5 tRNA methyltransferase 5homolog	1.11	−1.65	0.50
Syk	Spleen tyrosine kinase	1.19	−1.43	0.49
Irx5	Iroquois related homeobox 5	1.26	−1.29	0.48
Emr1	EGF-like modulecontaining, mucin-like	1.14	−1.42	0.43
Kcnip2	Kv channel interacting protein 2	1.11	−1.44	0.42
Loxl1	Lysyl oxidase like 1	1.18	−1.26	0.38
Col15a1	Procollagen XV	1.17	−1.27	0.38
Ssync	Syncoilin	1.27	−1.12	0.38
Arhgef19	Rho guanine nucleotideexchange factor 19	1.24	−1.14	0.36
Ptprc	Protein tyrosine phosphatase,receptor type C	1.05	−1.44	0.36
Aurka	Aurora Kinase A	1.26	−1.10	0.35
Rasl11b	RAS-like family 11member B	1.03	−1.49	0.35

Adult heart mRNA was collected from Gata6^{fl/fl-βMHC-cre} mice and compared against mRNA from Gata6^fl/fl to generate Affymetrix expression arrays of genes that were specifically changed in with deletion of Gata6. And identical protocol was used to analyze genes changed with deletion of Gata4 from the heart. Afterwards the two arrays sets were cross compared to examine genes that might be more specific to GATA-4 or GATA-6 regulation, which is represented as the column “difference” showing normalized expression results. The values represent the difference between normalized GATA-4 expression over normalized GATA-6 expression, both of which are corrected to the common cre control and each respective floxed line control. The negative numbers represent downregulated genes. Gene names are shown in the left column and protein names are shown in the very next column.