Table 1. Differentially regulated genes from microarray.
Gene symbol | Description | ΔGATA-6 | ΔGATA-4 | Difference |
Timp4 | Tissue inhibitor of metalloproteinase 4 | −1.18 | 3.00 | 3.56 |
Angptl7 | Angiopoietin-like 7 | 1.05 | 2.39 | 2.28 |
Spink4 | serine peptidase inhibitor, Kazal type 4 | 1.02 | 2.11 | 2.07 |
Gcnt1 | Glucosaminyl (N-acetyl) transferase 1 | −1.17 | 2.89 | 2.03 |
Ptgfr | Prostaglandin F receptor | −1.00 | 2.92 | 1.92 |
Avpr1a | Arginine vasopressin receptor 1A | 1.06 | 1.58 | 1.49 |
Zbtb16 | Zinc finger and BTB domaincontaining 16 | −1.17 | 2.10 | 1.24 |
Bhlhb9 | Basic helix loop helix domain containing class B9 | −1.05 | 2.18 | 1.23 |
Scgb1c1 | Secretoglobin 1C1 | −1.01 | 2.09 | 1.10 |
Gm129 | Gene model 129 | −1.10 | 2.00 | 1.09 |
Fmo2 | Flavin containing monooxygenase 2 | −1.10 | 1.83 | 0.92 |
Gsta3 | Glutathione S transferase, alpha 3 | −1.04 | 1.81 | 0.85 |
Adh1 | Alcohol dehydrogenase 1 | −1.28 | 1.61 | 0.83 |
Rhobtb1 | Rho related BTB domain containing 1 | −1.09 | 1.61 | 0.69 |
Tsc22d3 | TSC22 domain family 3 | −1.05 | 1.64 | 0.69 |
Wee1 | Wee 1 homolog | −1.06 | 1.57 | 0.63 |
Pfn2 | Profilin 2 | −1.02 | 1.61 | 0.63 |
Bc055107 | Family with sequence similarity 107, A | −1.13 | 1.51 | 0.63 |
Fstl4 | Follistatin like 4 | −1.37 | 1.34 | 0.61 |
Fkbp5 | FK506 binding protein 5 | −1.20 | 1.40 | 0.57 |
Gadd45g | Growth arrest and DNA damageinducible 45γ | −1.06 | 1.53 | 0.56 |
Bcl2l1 | BCL2 like 1 | −1.08 | 1.45 | 0.52 |
Ifi205 | Interferon activated gene 205 | −1.15 | 1.38 | 0.51 |
Tchhl1 | Trichohyalin like 1 | −1.02 | 1.45 | 0.47 |
Thrsp | Thyroid hormone responsiveSPOT14 homolog | −1.46 | 1.14 | 0.46 |
Il33 | Interleukin 33 | −1.03 | 1.42 | 0.45 |
Trdn | Triadin | −1.26 | 1.25 | 0.45 |
Ankrd10 | Ankyrin repeat domain 10 | −1.02 | 1.42 | 0.44 |
Ddit4 | DNA damage inducible transcript 4 | −1.02 | 1.42 | 0.44 |
Tom1l1 | Target of myb1 like 1 | −1.06 | 1.38 | 0.44 |
Cebpb | CCAAT/enhancer binding protein(C/EBP) beta | −1.08 | 1.35 | 0.42 |
Efna5 | Ephrin A5 | −1.04 | 1.38 | 0.42 |
Rgs2 | Regulator of G-protein signaling 2 | −1.01 | 1.41 | 0.42 |
Hey2 | Hairy/enhancer of split related withYRPW motif 2 | −1.35 | 1.09 | 0.35 |
Drd2 | Dopamine receptor 2 | 1.57 | −1.18 | 0.73 |
Nppb | Natriuretic peptide precursor type B | 1.55 | −1.04 | 0.59 |
Egr1 | Early growth response 1 | 1.19 | −1.62 | 0.58 |
Fbn1 | Fibrillin 1 | 1.26 | −1.41 | 0.55 |
Eg633640 | Predicted gene | 1.31 | −1.30 | 0.53 |
Rrad | Ras-related associated with diabetes | 1.00 | −2.09 | 0.52 |
Ptk2b | PTK2 protein tyrosine kinase 2 beta | 1.26 | −1.35 | 0.51 |
Trmt5 | TRM5 tRNA methyltransferase 5homolog | 1.11 | −1.65 | 0.50 |
Syk | Spleen tyrosine kinase | 1.19 | −1.43 | 0.49 |
Irx5 | Iroquois related homeobox 5 | 1.26 | −1.29 | 0.48 |
Emr1 | EGF-like modulecontaining, mucin-like | 1.14 | −1.42 | 0.43 |
Kcnip2 | Kv channel interacting protein 2 | 1.11 | −1.44 | 0.42 |
Loxl1 | Lysyl oxidase like 1 | 1.18 | −1.26 | 0.38 |
Col15a1 | Procollagen XV | 1.17 | −1.27 | 0.38 |
Ssync | Syncoilin | 1.27 | −1.12 | 0.38 |
Arhgef19 | Rho guanine nucleotideexchange factor 19 | 1.24 | −1.14 | 0.36 |
Ptprc | Protein tyrosine phosphatase,receptor type C | 1.05 | −1.44 | 0.36 |
Aurka | Aurora Kinase A | 1.26 | −1.10 | 0.35 |
Rasl11b | RAS-like family 11member B | 1.03 | −1.49 | 0.35 |
Adult heart mRNA was collected from Gata6fl/fl-βMHC-cre mice and compared against mRNA from Gata6fl/fl to generate Affymetrix expression arrays of genes that were specifically changed in with deletion of Gata6. And identical protocol was used to analyze genes changed with deletion of Gata4 from the heart. Afterwards the two arrays sets were cross compared to examine genes that might be more specific to GATA-4 or GATA-6 regulation, which is represented as the column “difference” showing normalized expression results. The values represent the difference between normalized GATA-4 expression over normalized GATA-6 expression, both of which are corrected to the common cre control and each respective floxed line control. The negative numbers represent downregulated genes. Gene names are shown in the left column and protein names are shown in the very next column.