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. 2013 Dec 31;8(12):e84569. doi: 10.1371/journal.pone.0084569

Figure 7. Stuctural requirements of the DNA targets.

Figure 7

In vitro BRET shift recorded between RXR-Luc and EYFP-RXR, or RXR-Luc and PPARG2-EYFP, or RXR-Luc and THR-EYFP in the presence of 100 nM of different responsive elements (described in Table 1). Values represent BRET measures (each in triplicate) integrated over a 10 min reading. (A), BRET shift obtained between 80 ku of RXR-Luc and 40 ku of PPARG2-EYFP with control (TE), single strand (ss) consensus DR1 and 13 different double strand DNA RE. (B), BRET shift obtained between 80 ku of RXR-Luc and 40 ku of THR-EYFP with control (TE), single strand (ss) consensus DR4 and 13 different responsive elements. (C), BRET shift between 80 ku luciferase of donor RXR-Luc and 40 ku fluo of acceptor EYFP-RXR in absence (control TE) or in presence of 100 nM ds DNA DR1 or DR4 RE. Values shown are means ± SD (n = 3). Statistical differences relative to control (TE) were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison post hoc test: ***P<0.001 and *P<0.05.