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. 2013 Dec 31;8(12):e85063. doi: 10.1371/journal.pone.0085063

Table 3. Newly designed primers used in this study.

Primer Name    Sequence (5'3')
Position (nt)    Size (bp) Target region Reference
ORF1-1F GTG AAT GAA GAT GGC GTC TA 1-20
ORF1-1R AGT CTT GGT AGG GCC TAA AG 697-716 716
ORF1-2F GTG TAG GAG AAT GAT CCA G 664-682
ORF1-2R GAG AGC TCC TCC TTC GC 1436-1452 789
ORF1-3F CCA AGT CTG CTT CAC CTG AC 1353-1372
ORF1-3R GGG TGT TTC CGT TCT TGT C 1976-1994 642
ORF1-4F ACT GAC GCT GGC TCC GCA 1948-1965
ORF1-4R ATC CTT GCT AGC GGT CTC CT 2547-2566 619 ORF1 In this study
ORF1-5F GTC AAG CGC ATG AAC ATA CAA G 2492-2513
ORF1-5R CTC CAG TTG GCC TCT TGA TGA 3285-3305 814
ORF1-6F GGC ATG ATC TTG GAA GAA GG 3236-3255
ORF1-6R ATG CTT GCG CGA ATG ACC 3882-3899 664
ORF1-7F GGC TGC CAA GAA AAC CAT C 3823-3841
ORF1-7R ACC TCA GAA AGT GCA CAG AG 4523-4542 720
ORF1-8F CCA ATG GAA TTC CAT CGC CC 4489-4508
ORF1-8R CGA CGC CAT CTT CAT TCA CA 5080-5099 611
ORF2-1F CGC AAT CTG GCT CCC AGT T 5060-5078
ORF2-1R GTG CAT CTG CCA TTC TGA CAC 5864-5884 825 ORF2 In this study
ORF2-2F GAG CAA TTG TAT ACG GCT CC 5823-5842
ORF2-2R CTG GAT TCT TCT ACG CCC AT 6691-6710 888
ORF3-2F GAA CTG GYA ATG GGC GTA 6682-6699 ORF3 In this study
ORF3-2R TGC AAG NNN NTN NNN TCA C 7473-7492 811