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All synthetics of candidate genes were inserted into the vector pcDNA3.1− by EcoR I /HindIII restriction sites in (A), (B) and (C). The double gene expression vector pcDNA3.1-N3D4 (D) was constructed based on pcDNA3.1-D4, with the insertion of pCMV-sN3-polyA DNA fragment by Bgl II/Mlu I restriction sites.
