Table 2. Differentially expressed proteins of AML14.3D10 in context to cell stress and cell survival, on rhSP-D treatment.
| Locus tag/ Functional category | Spot No. | Protein description | Biological Function | Increased/ Decreased |
|---|---|---|---|---|
| Cell Stress | ||||
| gi|4759212 | 8 | Tubulin-specific chaperone a | Tubulin complex assembly | Decreased |
| gi|8922762 | 9 | Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 | L-methionine salvage from methylthioadenosine (Oxidoreductase) | Decreased |
| gi|32483377 | 13 | Peroxiredoxin 3 isoform b | Mitochondrion-specific peroxidase | Decreased |
| gi|7546411 | 14 | Chain A, X-Ray Crystal Structure For Human Manganese Superoxide Dismutase, Q143a | Mitochondrial matrix superoxide dismutase | Decreased |
| gi|45768728 | 18 | Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 | Mitochondrial respiratory chain complex III | Decreased |
| gi|113422970 | 25 | PREDICTED: similar to Copper chaperone for SOD | Copper chaperone for superoxide dismutase (CCS) | Increased |
| gi|62088106 | 32 | Thioredoxin-like 1 | Oxidoreductase | Increased |
| gi|42794775 | 45 | Thioredoxin domain containing 5 isoform 2 | Thioredoxin domain containing protein | Increased |
| gi|386758 | 47 | GRP78 precursor | Cytoprotective function; induced in Endoplasmic stress | Increased |
| gi|6470150 | 48 | GRP78 precursor | Cytoprotective function; induced in Endoplasmic stress | Increased |
| gi|6470150 | 49 | GRP78 precursor | Cytoprotective function; induced in Endoplasmic stress | Increased |
| gi|3154294/7 | 52 | Chaperonin | Mitochondrial chaperone | Increased |
| gi|24234688 | 54 | Heat shock 70kDa protein 9B precursor | Molecular Chaperone | Increased |
| gi|21040386 | 55 | HSPA9 protein | Candidate tumor suppressor gene; control of cell proliferation; a chaperone | Increased |
| gi|112491363 | 71 | Chain A, Crystal Structure Of The Alpha Subunit Of Human S- Adenosylmethionine Synthetase 2 | Methionine Adenosyltransferase Activity | Increased |
| gi|5174529 | 72 | Methionine adenosyltransferase II, alpha | Synthesis of S-adenosyl-methionine | Increased |
| Survival and Inflammation related proteins | ||||
| gi|1633186 | 2 | Chain A, Pkci-1-Apo+zinc | Protein Kinase C Interacting (inhibitory) | Decreased |
| gi|123377 | 20 | High-mobility group I (HMGA1a) | Cell survival protein, oncogenic phenotype | Decreased |
| gi|62088704 | 56 | Heterogeneous nuclear ribonucleoprotein K isoform a variant | Cell cycle progression; induction of p53 genes | Increased |
| gi|118084547 | 60 | Aryl hydrocarbon receptor interacting protein | Signal transducer activity | Increased |
| gi|4505587 | 74 | Platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa | Lipid Metabolism | Decreased |
Proteins belonging to the categories (i) cell stress and (ii) inflammation and survival related proteins that showed altered expression (≥ 3-fold change) on treatment with rhSP-D (10µg/ml) in AML14.3D10 cells ( n=3). Spot number relates to the protein spots marked on the 2-DE gel images of proteins extracted from rhSP-D treated and untreated AML14.3D10 cells (Figure 3).