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. 2013 Dec 31;8(12):e85403. doi: 10.1371/journal.pone.0085403

Figure 2. Distribution of NAMPT protein in rat brain after MCAO.

Figure 2

(A-C) Representative images of double immunofluorescent staining of NeuN (for neuron) and NAMPT (A), Iba-1 (for microglia) and NAMPT (B), GFAP (for astrocyte) and NAMPT (C) in rat brain after 30 min MCAO and 24 h reperfusion. Immunofluorescent staining was repeated on three rats. (D) Using Western blot (upper panel) and immunochemistry staining (lower panel), the i.v. injected His-tagged NAMPT was detected in the ischemic brain area after 30 min MCAO and 24 h reperfusion.