Skip to main content
. 2013 Dec 31;8(12):e85403. doi: 10.1371/journal.pone.0085403

Figure 6. The effect of recombinant NAMPT on ischemic-induced TNF-α release in primary neuron-glial mixed culture.

Figure 6

Wild type NAMPT (WT) or H247A NAMPT (H247A) was applied 30 min before OGD. (A and B) TNF-α in the medium was determined at 24 h after NAMPT treatment (A) or after combined treatment with OGD (B). N=6. ***P<0.001, compared with E-S Glu (A) or E-S Glu-free (B), One-way ANOVA. (C and D) TNF-α in the medium was determined at 3, 6, 12 or 24 h after the treatment of NAMPT (C) or after combined treatment with NAMPT and OGD (D). N=5. *P<0.05, **P<0.01, ***P<0.001, compared with “0” of E-S Glu (C) or E-S Glu-free (D), One-way ANOVA. # P<0.05, ## P<0.01, ### P<0.001, compared with 0 h recovery in each group, One-way ANOVA. E-S is Earle’s solution; Glu is glucose.