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. 2013 Dec 31;8(12):e85403. doi: 10.1371/journal.pone.0085403

Figure 7. TNF-α neutralizing antibody protected neuronal injury induced by OGD and NAMPT in primary neuron-glial mixed culture.

Figure 7

NAMPT and TNF-α neutralizing antibody (5μg/ml, TNF-α Ab) was applied 30 min before OGD. (A) The percentage of necrotic cells in total cells determined by using PI and DAPI staining. (B) The percentage of necrotic neuron cells determined by using NeuN, PI and DAPI staining. N=5-6. *P<0.05, ***P<0.001, compared with no NAMPT no TNF-α Ab in control, One-way ANOVA. # P<0.05, ## P<0.01, ### P<0.001, compared with no NAMPT no TNF-α Ab in OGD, One-way ANOVA.