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. 2013 Dec 31;8(12):e85392. doi: 10.1371/journal.pone.0085392

Figure 2. Detection of endogenous TRPV2.

Figure 2

A, Western blot analysis with anti-TRPV2 2D6 of F11 cells treated with control siRNA or TRPV2 siRNA (100 nM, 48 h). Quantification of the band corresponding to the molecular weight of TRPV2 was measured using LiCor Odyssey software. TRPV2 band intensity was normalized to actin. Error bars represent S.E.M. from 3 separate experiments. B, Immunoprecipitation of TRPV2 with 10 µg of indicated antibodies from 2.5 mg mouse brain lysate and 2.5 mg mouse heart lysate. TRPV2 was detected by western blot with IR dye-labeled 2D6 antibody. Input represents 100 µg of total protein. Membranes from yeast overexpressing recombinant rat TRPV2 were loaded as a control.