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. 2013 Dec 31;8(12):e85392. doi: 10.1371/journal.pone.0085392

Figure 5. Effect of IGF-1 on endogenous TRPV2 trafficking in F11 cells.

Figure 5

A, F11 cells were treated with IGF-1 (20 ng/ml) for the indicated times and immunoblotted with a phospho-Akt specific antibody. Membranes were then stripped and re-probed with a pan-Akt antibody. B, Biotinylation of surface proteins from F11 cells was performed following the procedure from Figure 5B. C, F11 cells treated with vehicle (PBS) or IGF-1 (20 ng/ml) for 20 min were fixed and immunolabeled for TRPV2 (17A11 antibody). Images are representative of 3 separate experiments. Scale bar represents 10 µm.