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. 2013 Dec 31;8(12):e85660. doi: 10.1371/journal.pone.0085660

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© 2013 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PBMC were transfected with C- or T-bearing reporter vectors with or without pretreatment of over-expression HNF3γ plasmid. Left panel: Bar chart of alterations in relative luciferase activity. Right panel: Representative western blot (top) and bar chart (below) of alterations in HNF3γ protein levels either pretreated with over-expression HNF3γ plasmid or not. Renilla luciferase activity was measured and normalized to firefly luciferase. Data represent mean ± SD of at least three independent experiments. *P<0.05 vs. cells treated with either pGL3-basic-NOX4 (C) or pGL3-basic-NOX4 (T); ▲ P<0.05 vs. cells treated with over-expression HNF3γ plasmid plus pGL3-basic-NOX4 (T).