FIG. 4.

HGTD-P induces apoptotic cell death with caspase activation. PC-3 cells were transfected with 1 μg of mock vector, pcDNA-HGTD-P, or pcDNA-Bax. (A) After 48 h of transfection, cells were harvested, double stained with DAPI and anti-V5 antibody, and visualized with a confocal microscope. (B) Transfected cells with fragmented nuclei or chromatin condensation were counted at the indicated time points, and percent cell death was deetermined. (C) Cells were harvested at the indicated time points after transfection, and caspase assays were performed as described in the text. The caspase activities of mock-transfected cells were arbitrarily set at 1. The increase in caspase activity is presented.