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Phosphorylation of phospholamban levels are down-regulated in adult but not in paediatric HF patients. Total phospholamban (A), Ser16 (B), and Thr17 (C) phosphorylation levels were measured by western blot and normalized to calnexin. The measurement of total phospholamban was done on Ser16 and Thr17 blots. PLB, phospholamban; F, failing; NF, non-failing.
