TABLE 1.
Strains used in this study
| Strain | Genotypea | Reference or source |
|---|---|---|
| W303 | aho ade2 trp1 can1 leu2 his3 ura3 GAL psi+ | 32 |
| K1107 | aHOp-LacZ-HO 3′UTR | 25 |
| K5552 | α ASH1-myc | 15 |
| 10B | α HOp-ADE2-HO 3′UTR | 34 |
| YKEN104 | α HOp-ADE2-HO 3′UTR myo4Δ::CgHIS3 | This study |
| YKEN301 | α HOp-ADE2-HO 3′UTR kanMX6::GAL1p-KHD1 | 12 |
| YKEN303 | α HOp-ADE2-HO 3′UTR myo4Δ kanMX6::GAL1p-KHD1 | 12 |
| YKEN304 | α HOp-ADE2-HO 3′UTR ash1Δ | 12 |
| YKEN306 | α HOp-ADE2-HO 3′UTR myo4Δ ash1Δ | 12 |
| YKEN451 | α ASH1-myc myo4Δ::CgHIS3 | This study |
| YKEN9152 | α HOp-ADE2-HO 3′UTR mkt1-19 kanMX6::GAL1p-KHD1 | This study |
| YKEN9152a | α HOp-ADE2-HO 3′UTR mkt1-19 ash1Δ | This study |
| TTC4 | aHOp-LacZ-HO 3′UTR myo4Δ::CgLEU2 | This study |
| TTC46 | a/α HO/HO | 34 |
| TTC47 | α HOp-ADE2-ADH 3′UTR | 34 |
| TTC237 | α ASH1-myc mkt1Δ::CgHIS3 | This study |
| TTC478 | α HOp-ADE2-HO 3′UTR mkt1Δ::CgHIS3 | This study |
| TTC798 | α HOp-ADE2-HO 3′UTR pbp1Δ::CgHIS3 | This study |
| TTC848 | α HOp-ADE2-HO 3′UTR MKT1-HA::kanMX6 | This study |
| TTC929 | a/α HO/HO mkt1Δ::CgHIS3/mkt1Δ::CgHIS3 | This study |
| TTC993 | a/α HO/HO pbp1Δ::CgHIS3/pbp1Δ::CgHIS3 | This study |
| TTC996 | α HOp-ADE2-HO 3′UTR PBP1-HA::kanMX6 | This study |
| TTC999 | aHOp-ADE2-HO 3′UTR PBP1-HA::kanMX6 | This study |
| TTC1049 | aHOp-ADE2-HO 3′UTR PBP1-HA::kanMX6 MKT1-myc::kanMX6 | This study |
| TTC1085 | aHOp-LacZ-HO 3′UTR mkt1Δ::CgHIS3 | This study |
| TTC1089 | aHOp-LacZ-HO 3′UTR pbp1Δ::CgHIS3 | This study |
| TTC1163 | aHOp-LacZ-HO 3′UTR mkt1Δ::CgHIS3 pbp1Δ::CgHIS3 | This study |
| TTC1108 | α HOp-ADE2-ADH 3′UTR mkt1Δ::CgHIS3 | This study |
| TTC1233 | α HOp-ADE2-HO 3′UTR PBP1-HA::kanMX6 mkt1Δ::CgHIS3 | This study |
| TTC1234 | α HOp-ADE2-HO 3′UTR MKT1-HA::kanMX6 pbp1Δ::CgHIS3 | This study |
| TTC1257 | α HOp-ADE2-HO 3′UTR mkt1Δ::CgTRP1 pbp1Δ::CgHIS3 | This study |
| TTC1302 | aHOp-ADE2-HO 3′UTR PBP1-HA::kanMX6 MKT1ΔC-myc::kanMX6 | This study |
| TTC1313 | aho mkt1Δ::CgLEU2 | This study |
| TTC1316 | aho pbp1Δ::CgLEU2 | This study |
| TTC1319 | aho myo4Δ::CgLEU2 | This study |
| TTC1333 | α HOp-ADE2-ADH 3′UTR mkt1Δ::CgTRP1 pbp1Δ::CgHIS3 | This study |
| TTC1334 | α HOp-ADE2-ADH 3′UTR pbp1Δ::CgHIS3 | This study |
| PJ69-4A | atrp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ | 13 |
a
All strains except PJ69-4A were isogenic derivatives of W303.