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RID1 interacts with hTERT RT and C-terminal sequences. 35S-labeled protein fragments corresponding to different hTERT regions (N- and C-terminal regions and RT) (indicated by asterisks in the top gel) were coimmunoprecipitated with unlabeled Flag-tagged hTERT1-200 (bottom gel) in the presence (+) or absence (−) of wild-type (WT) hTR. Flag-hTERT1-200 (RID1) was detected by immunoblotting with anti-Flag antibody (αFlag). IP, immunoprecipitation.
