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. 2004 May;24(9):3757–3768. doi: 10.1128/MCB.24.9.3757-3768.2004

FIG. 1.

FIG. 1.

An evolutionarily conserved noncoding sequence resides in the upstream region of the HRC gene. (A) Schematic representation of the human HRC upstream region. The HRC upstream region (−2609 to +117) was cloned as a BamHI-Psp1406I fragment into CAT and β-galactosidase reporter plasmids such that transcription would start from the HRC transcriptional start site (red arrow) and translation would initiate in the reporter cDNA. Green box, evolutionarily conserved region; black arrow, HRC translational start site; B, BamHI; N, NcoI; Bg, BglII; P, Psp1406I. (B) Sequence alignment of the evolutionarily conserved element from the HRC upstream region from −608 to −468, relative to the transcriptional start site, in the human sequence. The box denotes the evolutionarily conserved MEF2 site.