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. 2004 May;24(9):3827–3837. doi: 10.1128/MCB.24.9.3827-3837.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 4. — Identification of a single CK-II phosphorylation site in the full-length FLAG-HOXA9 protein. (A) Phosphoamino acid analysis of the CK-II-phosphorylated ³²P-labeled His-tagged FLAG-HOXA9 protein indicated that the label was predominantly associated with serine. (B) Endoproteinase Glu-C peptides of CK-II-phosphorylated ³²P-labeled FLAG-HOXA9 separated by reverse-phase HPLC generated a single radioactive peak. (C) Edman sequencing of the HPLC radioactive fractions contributing to the peak released the label in cycle 9. (D) Sequence of the FLAG-HOXA9 protein with the predicted endoproteinase Glu-C and trypsin cleavage sites; Endoproteinase Glu-C peptide K167-K176 has S175 at position 9.