FIG. 1.

Effects of SSA1 and cochaperone mutations on [PSI⁺] phenotype. Cells were grown as colonies on YPD for 2 days at 30°C followed by 3 days at 25°C or as patches of cells replica plated onto −ade plates, which were incubated for 5 days at 25°C (−ade 25°) or for 3 days at the more stringent 30°C (−ade 30°). Representative areas of growth on plates are shown. Except for those in column 11, strains in upper panels have wild-type SSA1 and strains in lower panels have SSA1-21, as indicated. Strains in column 11 upper panels are ssa1Δ, and those in lower panels are ssa1Δ ssa2Δ. Relevant alleles of strains in each column are indicated; wt is wild type. Colors on YPD range from white to red, reflecting from most to least suppression of ade2-1 (see the text). The extent of ade2-1 suppression is also reflected as density of growth on −ade plates. Red cell color is enhanced on −ade plates compared with YPD plates, especially for nongrowing strains.