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. 2004 May;24(9):3928–3937. doi: 10.1128/MCB.24.9.3928-3937.2004

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FIG. 2. — Quantified levels of [PSI⁺]-mediated nonsense suppression. Strains carry plasmids expressing a translational fusion of Renilla (upstream) and firefly (downstream) luciferases, with or without a termination codon (UAA) early in the firefly coding region. The ratio of activities of firefly to Renilla enzymes for the UAA construct versus the control construct [(firefly/Renilla)_UAA/(firefly/Renilla)_control] provides a measurement of UAA read-through. Read-through values for mutant strains are expressed as percentages relative to values for the wild-type strain. Assays were done in triplicate at least three times. SSA1-21 derivatives of ssa2Δ and fes1Δ strains are not represented, since they are unable to propagate [PSI⁺].