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. 2004 May;24(9):3928–3937. doi: 10.1128/MCB.24.9.3928-3937.2004

FIG. 3.

FIG. 3.

Opposing effects of Sti1p and Fes1p on [PSI+]. (A) Increased expression of Sti1p and Fes1p. Patches or colonies of [PSI+] SSA1 and SSA1-21 transformants of a high-copy-number plasmid, with or without the indicated genes, were grown on selection plates with limiting adenine for 2 days at 30°C. While [PSI+] can propagate in SSA1-21 cells overproducing Sti1p, the enhanced weakening of [PSI+] makes it difficult to distinguish its presence at the level of individual colonies. (B) Patches of SSA1 and SSA1-21 cells, having or lacking FES1 as indicated, were replica plated onto plates lacking adenine and incubated at the indicated temperature (given in degrees Celsius) for 2 days.