FIG. 5.
Copurification of Hsp70-associated proteins. His6-tagged versions of Ssa1p and Ssa1-21p were purified on nickel affinity resin and subjected to Western analysis. Purified His6-tagged proteins are indicated at the top, and lane C is from a similar purification using lysates of cells expressing nontagged Ssa1p. The Hsp70 and Sti1p panels are from separate blots loaded with identical aliquots of the same samples. The Cpr7p and Ydj1p panels are from the Hsp70 and Sti1p blots, respectively, which were stripped and reprobed.