FIG. 6.

Relative abundance of Hsp70 and Hsp104. Abundance of Hsp70 and Hsp104 was examined by Western analysis. Blots probed with anti-Hsp70 antibodies were stripped and reprobed with anti-Hsp104 antibodies. Representative portions of membranes, stained by amido black as a loading and transfer control, are shown (Load). Relevant alleles of strains are indicated at the top; wt is wild type. The ssa1Δ ssa2Δ sample was diluted 1:3, as indicated, because Hsp104 abundance in this strain is very high (compare amounts in the Load panel). The signal in the Hsp70 blot for this strain represents Ssa3p and Ssa4p, which are expressed when Ssa1p and Ssa2p both are absent.