Figure 3.

CBD induces intracellular calcium influx in BV-2 cells. (a) CBD dose-dependently induces calcium influx in BV-2 microglial cells under serum-free conditions. The calcium traces are an average of three independent experiments each (n=15–30 single cells per experiment). (b) CBD-induced calcium influx is attenuated by the removal of extracellular calcium. The calcium traces are an average of three independent experiments each (n=15–30 single cells per experiment). (c) A representative trace (average of n=15 cells) showing the long-term effects of CBD on the intracellular calcium concentration. Treatment with CBD induces a transient intracellular calcium increase, followed by a delayed large calcium increase