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Flow cytometry dot plots of different cell populations in young SCs (left) and aged SCs (right) cultured for 4, 24, 48, and 72 h, as indicated. Early apoptotic cells (AnnV+/PI−, quadrant D4) can be discriminated from viable cells (AnnV−/PI−, quadrant D3), late apoptotic cells (AnnV+/PI+, quadrant D2), and necrotic cells (AnnV−/PI+, quadrant D1), according to their fluorescence emission
