Table 1. Annexin V/PI detection in flow cytometry of apoptotic and necrotic/late apoptotic cells in satellite cells of young and aged subjects.
Time |
Apoptotic and necrotic/late apoptotic satellite cells |
|||||
---|---|---|---|---|---|---|
(h) |
Young satellite cells |
Aged satellite cells |
||||
AnnexinV+/PI– | AnnexinV+/PI+ | AnnexinV–/PI+ | AnnexinV+/PI– | AnnexinV+/PI+ | AnnexinV–/PI+ | |
4 | 14.4±1.9* | 2.4±1.0 | 2.9±1.9 | 28.2±5.4 | 4.0±1.6 | 1.4±0.6 |
24 | 15.5±3.0* | 3.4±1.4 | 2.9±1.2 | 33.1±7.3 | 3.4±0.7 | 2.5±0.9 |
48 | 14.4±4.5** | 0.9±0.4 | 1.8±0.5 | 27.8±3.5 | 2.0±0.7 | 1.4±0.3 |
72 | 16.8±7.3** | 0.9±0.5 | 0.7±0.5 | 33.7±4.8 | 1.5±0.8 | 0.8±0.4 |
AnnexinV+/PI−: young SCs versus aged SCs: *P<0.05; **P<0.01