Figure 4.

RIPK3 activation and its requirement in ARPE-19 cell death in response to oxidative stress. (A) Sequential images showing the distribution and aggregation of RIPK3 in ARPE-19 cells transfected with RIPK3-GFP expression plasmid and treated with 300 μM H₂O₂ (a–e) or 150 μM tBHP (f–j) for indicated times. Scale bar equals 25 μm. The arrows denote the RIPK3 aggregates in the treated cells. (B) Knockdown of RIPK3 by transfection with RIPK3 siRNAs shown by real-time qPCR. ***P<0.001. (C) Rescue of RPE cell death in RIPK3 siRNA-transfected cells at 24 h after H₂O₂ (300 μM) or tBHP (150 μM) treatment. *P<0.05. Results from 500 μM H₂O₂ are not statistically significant