FIG.5.

(A) Rescue of tracheal and mesodermal cell migration by altered versions of the Dof600 protein. The lumen of the tracheal system of embryos was visualized with the 2A12 antibody in red (subpanels a to c and a′ to c′) or in brown (subpanels a" to c"), terminal cells were visualizedwith the anti-DSRF antibody in green (subpanels a′ to c′) and the pericardial cells with anti-Evenskipped antibodies in brown (subpanels a" to c"). Confocal projections are shown for a representative rescued embryo expressing the following transgenes in a dof^−/− background under the control of the btlGal4 driver: UASdof600 (subpanels a and a′), UASdof600Y515F (b and b′) and UASdof600Y486F (subpanels c and c′). Sections are shown for representative rescued embryos expressing the following transgenes in a dof^−/− background under the control of the twiGal4 driver: UASdof600 (subpanel a"), UASdof600Y515F (subpanel b"), and UASdof600Y486F (subpanel c"). (B) Tyrosine 515 serves as a Csw binding site. S2 cells cotransfected with csw and the different forms of V5-tagged dof600 as indicated on the figure, with or without myc-btl^act, were lysed after 8 h of induction with 0.6 mM CuSO₄. Whole-cell lysates were immunoprecipitated with anti-Csw antibodies, subjected to SDS-PAGE and immunoblotted with anti-V5 antibodies (upper panel) and anti-Csw antibodies (bottom panel).