Figure 2.

Validation of mouse IL-6 as direct miR-365 target. (a) Alignment of miR-365 and its target sites in intact or mutated IL-6 3′-UTR. (b) Schematic representation of pprime-dsRed-miR-365 construct. (c) Normalized luciferase activities of IL-6 3′-UTR renilla luciferase reporter plasmid, and IL-6 3′-UTR-mutant renilla luciferase reporter plasmid, 48 h after co-transfection together with pprime-miR-365 or empty vector in HEK293 cells. (d) Western blotting with anti-IL-6 antibody of total lysates from empty vector and pprime-miR-365-infected microglial cells, at 96 hours post virus transduction. β-actin was used for protein normalization