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IL-6 downregulation in SOD1-G93A mouse microglia. (a) Semiquantitative RT-PCR using specific primers for IL-6 and β-actin mRNAs, on total RNA from nt and SOD1-G93A microglia. β-actin was used for normalization. (b) Western blotting with anti-IL-6 antibody on total lysates from nt and SOD1-G93A microglia. β-actin was used for protein normalization. (c) IL-6 levels in the culture media of microglia from nt and G93A mice, after 6 h incubation in fresh media, as assessed by ELISA
