Figure 4.

MiR-125b-mediated STAT3 downregulation in SOD1-G93A mouse microglia. (a) The two minimum free energy duplexes of miR-125b-5p and 3′-UTR of mouse STAT3 (NM_011486.4) as predicted by RNA hybrid. (b) Western blotting with anti-STAT3 antibody of total lysates from empty vector and pprime-miR-125b infected microglia at 96 hours post virus transduction. β-actin was used for protein normalization. (c) Semiquantitative RT-PCR using specific primers for STAT3 and β-actin mRNAs of total RNA from nt and SOD1-G93A microglia. β-actin was used for normalization. (d) Western blotting with anti-STAT3 antibody of total lysates from nt and SOD1-G93A microglia. β-actin was used for protein normalization