Figure 5.

oAβ-induced IL-1β release is dependent on ROS. LPS-primed microglia were treated with varying doses of NAC (0.1–10 μM) for 30 min before oAβ stimulation, and IL-1β (a) in the culture supernatant as well as caspase-1 activity (b) were measured at 48 h. Data indicate means±S.D. for three independent experiments. ***P<0.001, versus LPS-primed microglia (ctl). †, ††, or ††† denotes P<0.05, 0.01, or 0.001, respectively, versus LPS priming+oAβ+NAC (c). LPS-primed microglia were treated with the ROS scavenger NAC (10 μM) for 30 min before oAβ stimulation and cellular (CM-H₂DCFDA) and mitochondrial (MitoSOX) ROS were assessed by flow cytometry. Data are one representative of three independent experiments