FIG. 2.

Products of DNase I digestion of nucleosomes without linkers. DNA or nucleosomes (5S Top as in Fig. 1; presented schematically at the bottom of the figure) reconstituted with histone octamers from chicken erythrocytes were mixed with 10 μM Nhp6 and 0.2 μM Spt16-Pob3 as indicated and then treated with 1×, 2×, 4×, 8×, or 16× DNase I as described in Materials and Methods. Products were separated by denaturing PAGE and then detected by autoradiography. Labels indicate the size of the product as described in Fig. 1. Sizes on the left indicate major cut sites observed with free nucleosomes, and those on the right indicate sites enhanced by addition of Nhp6 or yFACT. These sites are also denoted with circles in the center of the figure; open circles indicate regions partly affected by Nhp6 alone, and solid circles indicate effects that depend on yFACT.