FIG. 7.

Fem1c and R3 alleles exhibit similar patterns of expression. Ethidium bromide-stained agarose gels showing the results of RT-PCR for β-actin (control) and transcripts from the R3 (Fem1c/Rosa3) and Fem1c (exons 2 and 3 [Ex 2 + 3]) alleles in different tissues of R3/+ mice. Both alleles are expressed in brain, eye, and kidney but not in skeletal muscle (consistent with the X-Gal staining pattern shown in Fig. 1). The R3 allele is expressed in cardiac muscle, but only low levels of the Fem1c product were detected in this tissue. The primers used for these products were as described for Fig. 6.