Skip to main content
. 2004 May;24(9):3894–3906. doi: 10.1128/MCB.24.9.3894-3906.2004

FIG. 5.

FIG. 5.

Pdcd4 preferentially inhibits translation of 5′UTR structured mRNA in vitro. (A) Structures of 5′UTR stem-loop structured luciferase (pCMV-SL-LUC) and nonstructured luciferase (pCMV-LUC); (B) in vitro translation of 5′UTR structured and nonstructured luciferase. Rabbit reticulocyte lysate was preincubated with increasing amounts of Pdcd4 (0 to 2 μg) for 5 min at 30°C prior to the addition of the nonstructured luciferase mRNA (0.2 μg) (lanes 1 to 4) or 5′UTR structured luciferase mRNA (0.2 μg) (lanes 5 to 8). The translation was performed in a total volume of 20 μl as described in Materials and Methods. The band intensity was determined with a PhosphorImager. The value obtained for nonstructured or structured luciferase mRNA with 0 μg of Pdcd4 was designated as 100%.