Table 1E.3.1.

Adapter Oligonucleotide Sequences

Adapter	DNA sequence 5′ to 3′	Purification
AP-A_bc-ACACa	GTT CAG AGT TCT ACA GTC CGA CGA TCA CAC NN	PAGE
AP-B_bc-ACACb	5Phos/GTG TGA TCG TCG GAC TGT AGA ACT CTG AAC CTG TC/3Phos	PAGE

^aThe underlined sequence represents the barcode sequence (ACAC). The “NN” two-base random nucleotide overhang allows for ligation to the ends of MmeI-digested DNA fragments.

^bThe underlined sequence is the barcode sequence (GTGT; the complement of ACAC). The phosphorylated 5′-end allows for ligation to the dephosphorylated genomic DNA fragments. Note that AP-B_bc-ACAC is five nucleotides longer on the 3′ end than AP-A_bc-ACAC, which prevents adapter-adapter ligation. In addition, the 3′ phosphorylation of AP-B_bc-ACAC prevents the formation of adapter-adapter products.