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Blastocyst PCR genotyping. PCR products (1 to 10 of 17) from day 3.5 blastocysts are shown with the 100-bp fragment from a DNA ladder (L), negative control (H2O), wild-type control (+/+), and Rps19 targeting vector as a positive control (C). Amplification of the wild-type allele and the targeted allele resulted in 123- and 109-bp PCR products, respectively.
