TABLE 5.
Enzymatic kinetics of FEN1 on a double-flap substrate
| FEN1 protein | Km (nM) | Vmax (10−4 nM/s) |
|---|---|---|
| Wild-type Rad27p | 4.8 ± 0.8 | 27.0 ± 4.7 |
| Rad27-G67Sp | 8.6 ± 1.9 | 5.5 ± 1.0 |
| Rad27-G240Dp | 4.5 ± 0.2 | 2.3 ± 0.1 |
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