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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Chem Biol. 2013 Nov 14;20(12):10.1016/j.chembiol.2013.09.021. doi: 10.1016/j.chembiol.2013.09.021

Figure 4. Positive electrospray spectra of HHQ produced by a double pqsA- pqsH- mutant fed with various culture supernatants providing 2-ABA.

Figure 4

When a double pqsB−pqsL− mutant was cultivated in M9 mineral medium with (a) acetate labelled at position 1 or (b) at position 2 with 13C as carbon source and the supernatant added to a nonpolar pqsA- pqsH- mutant culture in TSB. (c) pqsB− pqsL− mutant cultivated in M9 medium with unlabelled acetate and the supernatant fed to a double pqsA- pqsH- mutant mutant grown in fully 13C acetate, (d) supernatant of a pqsC- mutant cultivated in TSB and fed to a pqsA- pqsH- mutant grown in TSB and supplemented with fully 13C-labelled octanoic acid.