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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Methods. 2013 Nov 17;11(1):10.1038/nmeth.2731. doi: 10.1038/nmeth.2731

Figure 3.

Figure 3

Holidic diet as a tool to study behavior (a, b) Activity and sleep profiles of flies on holidic and oligidic diets (a) (summed into 30 minute bins) for female and male (Supplementary Fig. 4) flies recorded for 72 h following a 24 h acclimation period. Black and white boxes represent light and dark periods. (b) Average sleep amount and total waking activity were summed during the 72 h recording. They did not significantly differ between diets (P > 0.35, n = 16, for both comparisons, Wilcoxon rank-sum test). Plotted data represent mean ± s.e.m. (c) Diet preference test. After maintenance on holidic diets lacking either sucrose or amino acids for a period of 3 or 6 days, flies were then assayed for their choice of sugar alone or yeast alone. Flies deprived of amino acids showed a significantly greater yeast preference (Yeast Preference Index; YPI) than those deprived of sugar (P < 0.0001, n = 13 for 3-day deprived and n = 14 for 6-day deprived flies, Wilcoxon rank-sum test).