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. 2004 May;24(9):3596–3606. doi: 10.1128/MCB.24.9.3596-3606.2004

FIG. 1.

FIG. 1.

TFIIIC activates transcription of SNR6 chromatin to a high level. (A) The yeast U6 snRNA gene. Locations of important elements are indicated relative to the transcriptional start site (bent arrow) as +1. (B) The scheme of the experiment. TFIIIC was added at the beginning (a) or near the end (b) of chromatin assembly, and supplementary addition (TFIIIC) was made at assembly of promoter complexes (c) as specified in panels C and D. rNTPs, ribonucleoside triphosphates. (C) Transcription of naked DNA (lanes 2 and 3) or chromatin (lanes 1 and 4 to 8) was carried out without or with TFIIIC as outlined in panel B and indicated above each lane. The 98-nt primer extension product of the accurately initiated U6 transcript and the recovery marker are identified at the left side. Fold increase of transcript yield in each lane after normalization with respect to repressed chromatin (lane 1) is given at the bottom of the panel. (D) Quantitative comparisons: fold increase of transcript yield relative to lane 2 (naked DNA without TFIIIC), normalized for sample recovery. Standard deviations of three independent experiments are specified.