FIG. 4.

Structure of SNR6 gene chromatin downstream of box B. The partial MNase digestion pattern for naked DNA (lanes 1 to 8) or chromatin (lanes 9 to 16) with (lanes 5 to 8 and 13 to 16) or without (lanes 1 to 4 and 9 to 12) TFIIIC is shown. Positions of box A, the terminator (T), and box B are marked at the right side. The gray ellipse to the left spans the positioned nucleosome, and the black box spans protection due to TFIIIC. Asterisks denote hypersensitive sites flanking the TFIIIC-protected box B in chromatin, but not in naked DNA. The 3′ end of the primer extension probe was located 174 bp from the 3′ end of box B, complementary to the top strand, and was also used for the sequencing ladder.